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Figure S2. Morphological characterization of Tctp knockdowns. (A and B) Micrographs of stage 39 con-MO- or tctp-MO-injected embryos. Albeit with smaller eyes, Tctp morphants appear morphologically normal, and their development progresses at comparable rates relative to con-MO-injected tadpoles. (C) Plot of eye size measurements (mean ± SEM; ** P < 0.0063, unpaired t-test). (D) Plot of body length (anteroposterior axis) measurements (mean ± SEM; n.s. P < 0.8614, unpaired t-test). (E) The normalization of Tctp protein expression levels was evaluated by Western blot after a morpholino-resistant tctp transgene was co-injected with tctp-MO. Protein extracts were prepared from stage 37/38 embryos. (F) Complements Fig. 2I: Summary of the statistical analysis examining the effect of normalizing Tctp expression levels on the development of Tctp-depleted projections. (G) Complements Fig. 3D-H: Summary of the statistical analysis performed to evaluate the impact of Tctp deficiency on the length of the retinotectal projection. Scale bars: 110 μm. Image published in: Roque CG et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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