XB-IMG-152494
Xenbase Image ID: 152494
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Figure S3. Tctp deficiency impairs axon extension in vivo. (A and B) Coronal sections of stage 43 control or Tctp-depleted retinas probed for Glutamate decarboxylase (GAD65/67) and Calretinin, two cell-type-specific markers (GAD65/67 and Calretinin antisera detect GABAergic amacrine cells, and retinal ganglion cell/bipolar cells, respectively). (C and D) TUNEL staining on stage 43 control or Tctp-depleted retinas. The number of TUNEL-positive cells in the ganglion cell layer was found to be increased in Tctp morphants (mean ± SEM; n > 30 embryos for both backgrounds; *** P < 0.0001, unpaired t-test). (GCL: ganglion cell layer, approximated by the dashed outline). The boxed areas are shown at a higher amplification in the bottom panels. (E) Plot showing the average extension rates measured from time-lapse recordings of RGC axons coursing normally (i.e. without guidance errors) through the ventral optic tract (VOT) and dorsal optic tract (DOT) in controls and Tctp morphants (mean ± SEM; n = no. of axons analysed; VOT: *** P < 0.0001, unpaired t-test; DOT: * P < 0.0159, unpaired t-test). Scale bars: 50 μm. Image published in: Roque CG et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |