XB-IMG-152499
Xenbase Image ID: 152499
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Figure S8. Axonal Tctp interacts with Mcl1-related Bcl-XL. (A) Cultured rat cortical neurons (E18.5 + 3DIV) stained for Bcl-XL, and counterstained with Phalloidin. (B) PLA signal obtained using anti-Tctp and anti- Bcl-XL sera in cultured rat cortical neurons (E18.5 + 14 DIV), counterstained with DAPI and phalloidin. (C) Co-delivery of tctp-MO and tctp40-172 mRNA, encoding a truncated Tctp protein devoid of anti-apoptotic activity, fails to rescue the effects of Tctp depletion on the development of the retinotectal projection. Number of embryos displaying axon extension defects (‘tctp-MO’ versus ‘tctp-MO + tctp40-172 mRNA’: n.s., P = 1.00; Fisher’s exact test, performed on number of observations but plotted as percentages). Scale bars: 25 μm in (A), 10 μm in (B). Image published in: Roque CG et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |