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Figure 4. Tension on ectoderm and cells behavior during morphogenesis.(a) Time-lapse images of an embryo expressing ActTS-GR at gastrula, early neurula, and neurula stage (left), and its FRET ratio images (right). Vegetal view. Dashed line delineates the closing posterior neural plate (NP) and the lateral epidermis (LE). A: anterior. P: posterior. (b) Image of an embryo expressing hiActTS-GR at neurula stage (left) and its FRET ratio image (right). (c) Quantification of the FRET ratio in NP (oval with * in a) and LE (oval with ** in a) at the neurula stage. Values are average ± SD, n > 5. (d) Amplified image of NP (rectangle with * in a), and LE (rectangle with ** in a). Scale bars = 200 μm in a, b, 20 μm in d. *p < 0.05. Image published in: Yamashita S et al. (2016) Copyright © 2016, Macmillan Publishers Limited. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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