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Figure 5. Effects of C‐157 and D‐572 on apoptosis and viability of SAS cells. (a, b) Apoptosis and viability of SAS cells after treatment with chemicals. The results from triplicate samples are shown as the means ± SD. (b, right panels) SAS cells were labeled with Hoechst 33342, and treated with chemicals. Arrows indicate representative apoptotic cells containing condensed or fragmented nucleus. (c) Western blot analysis of SAS cell lysates with antibodies against phosphorylated and total Erk and Akt. The intensity of each band was measured by ImageJ software and the relative ratio of phosphorylated‐to‐total Erk or Akt was calculated. Image published in: Tanaka M et al. (2016) © 2016 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View

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