Xenbase Image ID: 152856

Figure 1. A: Western blot analysis of endogenous lin28a and lin28b expression in embryos injected with a total of 12.5 ng/embryo of lin28 MOs in the compound knockdown compared with CMO‐injected and uninjected control embryos at stage 16. GAPDH was used as a loading control. B: Scale diagram showing the genomic organisation of Xenopus mir‐17∼92 and mir‐106∼363 clusters. Coloured boxes indicate pre‐miRNA sequences, with each colour corresponding to paralog groupings based on seed sequence. Where known, the black and grey boxes, respectively, represent the major and minor forms derived from a common precursor. C: qRT‐PCR was performed on embryos injected with 10 ng each/embryo of lin28a1, a2 and b MOs and control embryos, at stage 10.5. Fold change in expression of miRNAs is shown compared with controls and normalised using U6 by the 2‐ΔΔCt method. Fold change is given as average of 3 biological replicates, with error bars representing SE. D: Predicted secondary structure of Xenopus pre‐mir‐363. The sequences of mature mir‐363‐5p, mir‐363‐3p, and the putative lin28 binding site are indicated. Image published in: Warrander F et al. (2016) © 2015 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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