Xenbase Image ID: 153004

Fig. 2. Dorsal depletion of hace1 leads to multiple developmental defects. a Immunoblot analysis shows that HACE1 MO inhibited translation of hace1-myc mRNA but not that of myc-hace1 mRNA used for rescue experiments. Xenopus laevis embryos were injected with the indicated sets of MOs (30 ng) and mRNAs (1.2 ng). α-tubulin was a loading control. b Embryos were injected with 30 ng of HACE1 MO alone (middle panel) or 30 ng of HACE1 MO plus 1.2 ng of myc-hace1 mRNA (right panel) into the animal region of two dorsal blastomeres at the four-cell stage. c, d Embryos were classified into severe, mild, and normal groups on the basis of the extent of each defect. *P < 0.05 (with Bonferroni correction), Mann–Whitney U-test. c N, number of total right and left sides of embryos we evaluated. The right side and left side of each embryo were separately evaluated. d N, number of total eyes we evaluated. The right eye and left eye of each embryo were separately evaluated. e, f The body length of embryos at stage 40-41 was quantified (Uninjected, n = 59; HACE1 MO, n = 53; HACE1 MO + myc-hace1 mRNA, n = 55). Values are expressed as the mean ± s.d. ***P < 0.001 (with Bonferroni correction), unpaired t-test Image published in: Iimura A et al. (2016) © The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page