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Fig. 3. hace1 knockdown inhibits convergent extension. a Keller sandwich explants were prepared from embryos injected with 30 ng of control MO or HACE1 MO into dorsal blastomeres at the four-cell stage. HACE1 MO (middle panel, n = 18), but not control MO (left panel, n = 18), caused a defect in elongation of explants. The defect in elongation caused by HACE1 MO was partly rescued by coinjection of 1.2 ng of myc-hace1 mRNA (right panel, n = 18). b The length-to-width ratio of explants was quantified. Values are expressed as the mean ± s.d. *P < 0.05, unpaired t-test Image published in: Iimura A et al. (2016) © The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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