XB-IMG-153177
Xenbase Image ID: 153177
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Fig. 2 – Tissue-specific targeting of GFP-APP fusion proteins
in transgenic Xenopus. (A) Fluorescent images of tadpoles
microinjected in the first-cell stage with the constructs
depicted below the images. No fluorescence was observed
using the neural tubulin gene promoter (pNtub: upper panel),
while tail muscle fluorescence was found when the EF1α
gene promoter (pEF1α: middle panel) was used and tail
and jaw muscle fluorescence using the cardiac actin
promoter (pCac: lower panel). (B) Western blot analysis of
tail lysates of wild-type tadpoles and tadpoles transgenic for
GFP-APP and expressing the fusion protein in muscle cells.
Proteins were resolved on a 10% SDS-PAGE gel and the
anti-APP antibody C87 was used. Molecular weight markers
(Mr) are indicated on the right. SP: signal peptide sequence;
TM: transmembrane domain; pA: poly-adenylation signal. Image published in: Collin RW and Martens GJ (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |