XB-IMG-153180
Xenbase Image ID: 153180
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Fig. 5 – Identification of the region within Xenopus APP cDNA responsible for its aberrant promoter activity. (A) 5′-end deletion
mutants of the APP-GFP construct were used in transgenesis and the generated animals were screened for fluorescence (fluo) in
the brain (+: fluorescence, −: no fluorescence). The linear fragments depicted were produced via digestions with the indicated
restriction enzymes. The region within APP cDNA encoding the transmembrane domain is indicated by a dashed box. pA:
poly-adenylation signal. (B) Sequence analysis of the 5′-RACE PCR products to determine the start site of the aberrant transcript
(indicated by an asterisk). The sequence of the nested adapter oligo used to perform the 5′-RACE PCR is underlined. (C) Western
blot analysis of brain lysates of wild-type tadpoles (lane 1; background bands), tadpoles transgenic for 5′-deleted (microinjected
with NaeI/PauI digested APP-GFP construct) ΔAPP-GFP (lane 2; presence of a 32-kDa product not present in lane 1) or animals
expressing GFP alone (lane 3; 30-kDa GFP-product). Proteins were separated on a 12.5% SDS-PAGE gel and an anti-GFP antibody
was used. Molecular weight markers (Mr) are indicated on the left. Image published in: Collin RW and Martens GJ (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |