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Fig. 4. Whole-mount in situ hybridization analysis. Xfz8-Mo (20 ng) with b-galactosidase mRNA (300 pg) were injected into one lateral side of albino embryos. These embryos were raised until stage 33 and b-galactosidase activity was visualized with Red-gal substrate as a tracer. The embryos were then subjected to whole-mount in situ hybridization with probes, Xlim-1 (A), Xpax-2 (B), Claudin (C) or Na+, K+-ATPase (D). The expression of Xlim-1, Xpax-2, Claudin or Na+, K+-ATPase was not reduced in the Xfz8-Mo-injected side but several embryos showed massive staining in the tubule region indicated by an orange arrowhead in (B). Black arrowheads indicate three nephrostomes and arrows indicate pronephric duct. Image published in: Satow R et al. (2004) Copyright © 2004. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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