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XB-IMG-153186

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Fig. 5. Transverse sections of the pronephros. Embryos after 4A6 and 3G8 staining were sectioned. The apical surface of pronephric tubule structures was stained with 3G8 (red arrow). The entire cell surface of the duct structure was stained positive with 4A6 (red arrowhead). Unstained duct structures were observed in the Xfz8-Mo-injected side (B, arrowheads). In the Xfz8-Mo + d7n-Xfz8/pCS2-injected side, 4A6 staining was partially rescued (C). (D–F) Hematoxylin and eosin staining was performed on the same section of (A, B, and C), respectively. (A,D) Normal duct and tubule cells were cubic in shape and were arranged regularly. (B,E) In the Xfz8-Mo-injected side, duct and tubule cells were circular in shape and arranged irregularly. (C,F) Co-injection of d7n-Xfz8/pCS2 partially rescued the defects caused by Xfz8-Mo. (G–I) Duct structures in the uninjected, Xfz8-Mo-injected or Xfz8-Mo + d7n-Xfz8/pCS2-injected side of a section at the caudal position, respectively. (J–L) Hematoxylin and eosin staining was performed on the same caudal section (G, H, or I), respectively. (G,J) Normal duct structure. (H,K) Duct cells in the Xfz8-Mo-injected side were circular in shape and arranged irregularly. (I,L) Co-injection of d7n-Xfz8/pCS2 partially rescued the defects caused by Xfz8-Mo.

Image published in: Satow R et al. (2004)

Copyright © 2004. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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