XB-IMG-153189
Xenbase Image ID: 153189
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Figure 6.
Axis Duplications in Xenopus Embryos Injected Ventrally with ΔRGS and in Mouse Embryos Homozygous for the Loss-of-Function AxinTg1 Allele
(a and b) Xenopus embryos with axis duplications caused by injection of 2 ng ΔRGS in one ventral blastomere (a) or 1 ng ΔRGS in two ventral blastomeres (b). The embryo in (b) is also strongly dorsalized. Scale bars, 0.5 mm.
(c) Frequency of axis duplications in embryos injected with Axin, ΔRGS, or ΔRGS together with Axin or C-cadherin.
(d) Ectopic expression of dorsal markers in embryos injected ventrally with ΔRGS. Each column shows the RT-PCR analysis of the dorsal (D) or ventral (V) halves of a pool of embryos. In uninjected embryos, Siamois, Goosecoid, Chordin, and Xnr3 are expressed dorsally. Ventral injection of ΔRGS, but not Axin, induces ectopic expression of the four dorsal markers.
(e–g) Lateral view of a normal E7.5 mouse embryo (e) and two E8.5 AxinTg1/Tg1 embryos with axial duplications (f and g), visualized by in situ hybridization to HNF-3β, a marker of anterior axial mesoderm ( Sasaki and Hogan 1994). White arrows, primary axes; black arrowheads, ectopic axes. Scale bars, 0.2 mm. Image published in: Zeng L et al. (1997) Copyright © 1997. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |