Xenbase Image ID: 153198

Figure 1. Size-Fractionated Poly(A)’ RNA from LiCl Embryos and N-CAM Induction (A) Size-fractionated poly(A)t RNA from LiCl embryos. Sixty micrograms of poly(A)t RNA from LiCl embryos was size fractionated on a lO%-30% sucrose gradient in the presence of methylmercuric hydroxide. One-twentieth of each of the 16 fractions collected was electrophoresed on a formaldehyde-containing agarose gel. Nylon filter blots were made from the gel and probed with a “P end-labeled deoxythymidine 17-mer. An autoradiograph of the resulting blot is shown. (6) N-CAM induction by size-fractionated LiCl embryo poly(A)t RNA. Four-cell stage UV-ventralized embryos were microinjected with approximately 10 ng of size-fractionated poly(A)+ RNA. Total RNA was prepared from pooled embryos (32-62 per pool) at the tailbud stage. The RNA equivalent of 2.5 embryos was electrophoresed on agarose/formaldehyde gels. Nylon filter blots were probed both for N-CAM and for EFl-a mRNAs. RNA prepared from UV-ventralized embryos microinjected with RNA from fractions 7-9 is shown. Also shown is RNA from noninjected UV-ventralized and normal embryos. Image published in: Smith WC and Harland RM (1991) Copyright © 1991. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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