Xenbase Image ID: 153201

Figure 4. In Situ Hybridization to Normal, LiCI-, and UV-Treated Embryos Plain arrows indicate the edge of the blastocoel; arrowheads indicate the dorsal lip of the blastopore (or the lip of the blastopore for LiCl and UV embryos); open arrows show the anterior limit of the archenteron. (A) Stage 8 blastula; no staining. (6) Stage 9-10 blastula. Dorsal is at the top. Nuclei around the marginal zone are stained. The dorsal quadrant lacks detectable staining (limits of staining region shown with bars). Some background staining is evident in the blastocoel of (B) and (D) (see Harland, 1991). (C) Stage 11 gastrula. Dorsal is at the top. Staining is limited to the ventral side in the marginal zone. Lateral views of such embryos are consistent with the sectioned embryo presented in Christian et al. (1991 b). (D) Stage 12 late gastrula, lateral view. The anterior limit of the archenteron is indicated. Staining is absent from the yolk plug and endoderm, but otherwise extends in two “wings” around the closing blastopore. (E) Stage 14 early neurula, lateral view. The blastocoel collapses during neurulation. Staining is limited to the posterior ventral tissue. (F) Stage 16 neurula, dorsal view. Head is to the left. Arrowhead points to the remnant of the blastopore. Photograph is in the plane of the notochord, which extends between the arrowhead and the open arrow. Staining is excluded from the mesoderm below the neural plate, but residual staining is widespread in the posterior ventral mesoderm. Two patches of staining (one of which is marked with a square), are evident lateral to the anterior neural plate. (G) Head of stage 36 tadpole. Staining cells are visible next to the x. (H) Stage 11 gastrula from LiCI-treated batch of embryos. The blastopore (arrowhead) closes vigorously in such embryos, leading to a puckered appearance. No staining is evident (compare with [Cl). (I) Vegetal view of a stage 11 UV-treated gastrula. The blastopore appears late in UV embryos. Staining surrounds the marginal zone of this embryo (compare with [Cl). Image published in: Smith WC and Harland RM (1991) Copyright © 1991. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue wnt8a.L wnt-8, wnt8, Xwnt-8, Xwnt8 X. laevis Throughout NF stage 11 ventral marginal zone lower blastopore lip wnt8a.L wnt-8, wnt8, Xwnt-8, Xwnt8 X. laevis Sometime during NF stage 12 to NF stage 16 ventral circumblastoporal collar involuted ventral mesoderm Image source: Published Larger Image Printer Friendly View

Return to previous page