XB-IMG-153202
Xenbase Image ID: 153202
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Figure 5. Xwnt-8/B-Galactosidase mRNA Coinjection
(A-C) Animal (tier 1) (A), marginal (tier 2 or 3) (S), and vegetal (tier 4) (C) blastomeres of normal stage 6 embryos were microinjected with 0.5 ng
of f%galactosidase mRNA.
(D-G) Injections of mRNAs into UV-ventralized stage 6 embryos. (D) Marginal blastomeres with 0.5 ng of 6-galactosidase mRNA. (E) Animal
blastomeres with 0.5 ng of P-galactosidase plus 10 pg of Xwnt-6 mRNAs. (F) Marginal blastomeres with 0.5 ng of 6-galactosidase plus 10 pg of
Xwnt-8 mRNAs. (G) Vegetal blastomeres with 0.5 ng of 6-galactosidase plus 10 pg Xwnt-8 mRNAs. Figure shows slightly hyperdorsalized embryo
with bent axis.
(H and I) Injection of mRNAs into UV-ventralized stage 8 embryos with 0.5 ng of 5-galactosidase plus 10 pg of Xwnt8 mRNAs into marginal (H)
or vegetal (I) blastomeres. Image published in: Smith WC and Harland RM (1991) Copyright © 1991. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |