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Fig. 1. Notch signaling promotes formation of floor plate and hypochord and represses notochord. Notch signaling was repressed by injection of mRNA encoding Su(H) DNA-binding mutant (SDBM, B, E, H, K, N) and activated by Notch intracellular domain mRNA (NICD, C, F, I, L, O). Floor plate development was assayed by expression of Netrin (A–C), F-spondin (G–I), and FoxA2 (J–L). Notochord formation was assayed by Axial protocadherin (AxPC, D–F) and Tor70 staining (brown, G–O). Tor70 staining has high variability and background, and thus should not be considered quantitative. Hypochord development was assayed by F-spondin (G–I, staining ventral to notochord) and VEGF (M–O). (A–F) Dorsal views, anterior up. (G–O) Transverse vibratome sections through middle of spinal cord at tailbud stages. Image published in: Peyrot SM et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue ntn1.L netrin 1, Netrin-1, ntn1l, unc-6 X. laevis Throughout NF stage 17 neural plate floor plate neural tube pcdh1.L axial protocadherin, axpc X. laevis Throughout NF stage 14 notochord neural plate spon1.S F-spondin X. laevis Throughout NF stage 35 and 36 hypochord floor plate neural tube foxa2.L HNF-3B, hnf3-beta, hnf3b, hnf3beta, LOC108717763 X. laevis Throughout NF stage 35 and 36 floor plate vegfa.L vegf, vegf-a, vegfa-a, vegfa-b X. laevis Throughout NF stage 28 hypochord Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + dnrbpj + NF17 (in situ hybridization) fig.1.b Expression Phenotype decreased amount ntn1.L expression in floor plate Xla Wt + canotch1 + NF17 (in situ hybridization) fig.1.c Expression Phenotype mislocalised ntn1.L expression in neural plate increased amount ntn1.L expression in neural plate Xla Wt + canotch1 + NF14 (in situ hybridization) fig.1.f Expression Phenotype decreased amount pcdh1.L expression in notochord Xla Wt + dnrbpj + NF35/36 (in situ hybridization) fig.1.h Expression Phenotype decreased amount spon1.S expression in floor plate decreased amount spon1.S expression in notochord Xla Wt + canotch1 + NF35/36 (in situ hybridization) fig.1.i Expression Phenotype mislocalised pcdh1.L expression in axial mesoderm increased amount pcdh1.L expression in axial mesoderm Xla Wt + canotch1 + NF35/36 (immunohistochemistry) fig.1.i, l Anatomical Phenotype abnormal development of notochord Xla Wt + dnrbpj + NF35/36 (in situ hybridization) fig.1.k Expression Phenotype decreased amount foxa2.L expression in floor plate Xla Wt + canotch1 + NF35/36 (in situ hybridization) fig.1.l Expression Phenotype increased amount foxa2.L expression in axial mesoderm Xla Wt + dnrbpj + NF28 (immunohistochemistry) fig.1.n Anatomical Phenotype abnormal development of hypochord Xla Wt + dnrbpj + NF28 (in situ hybridization) fig.1.n Expression Phenotype decreased amount vegfa.L expression in hypochord Xla Wt + canotch1 + NF28 (immunohistochemistry) fig.1.o Anatomical Phenotype abnormal development of notochord Xla Wt + canotch1 + NF28 (in situ hybridization) fig.1.o Expression Phenotype mislocalised vegfa.L expression in axial mesoderm increased amount vegfa.L expression in hypochord Larger Image Printer Friendly View

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