Xenbase Image ID: 153359

Fig. S3. Loss of Shh signaling and Hh signaling cause similar developmental defects. (A) Control embryos at stage 42, lateral (A), dorsal, (A') and ventral (A") views. (B) Cylopamine treatment blocks all Hh signaling, resulting in reduced melanocytes, edema, and kinked tail (B), closely spaced eyes (B’), and gut-looping defects (B"). Injection of MOs designed to the translation start site (“Shh ATGMO,” C–C") and splice donor site (“Shh spliceMO,” D–D") show similar phenotypes. (E) Injection of Shh mRNA (pink, lacZ staining) results in excess melanocytes (D, arrow), ventralization of the eye (E, E'), and gut looping defects (E"). Image published in: Peyrot SM et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + Cyclopamine + NF42 (morphology) fig.S3. b Anatomical Phenotype abnormal bending of tail abnormally decreased number of melanophore edematous ventral trunk Xla Wt + Cyclopamine + NF42 (morphology) fig.S3. b^1 Anatomical Phenotype abnormally localised eye decreased size of the intercanthal region Xla Wt + Cyclopamine + NF42 (morphology) fig.S3. b^1^1 Anatomical Phenotype abnormal intestine morphology Xla Wt + shh MO + NF42 (morphology) fig.S3.c Anatomical Phenotype abnormal bending of tail abnormally decreased number of melanophore edematous ventral trunk Xla Wt + shh MO + NF42 (morphology) fig.S3.c' Anatomical Phenotype abnormal intestine morphology Xla Wt + shh MO + NF42 (morphology) fig.S3.c^1 Anatomical Phenotype abnormally localised eye decreased size of the intercanthal region Xla Wt + shh MO + NF42 (morphology) fig.S3.d Anatomical Phenotype abnormal bending of tail abnormally decreased number of melanophore edematous ventral trunk Xla Wt + shh MO + NF42 (morphology) fig.S3.d' Anatomical Phenotype abnormal intestine morphology Xla Wt + shh MO + NF42 (morphology) fig.S3.d^1 Anatomical Phenotype abnormally localised eye decreased size of the intercanthal region Xla Wt + shh + NF42 (morphology) fig.S3.e Anatomical Phenotype abnormally increased number of melanophore Xla Wt + shh + NF42 (morphology) fig.S3.e' Anatomical Phenotype abnormal development of alimentary system abnormal intestine morphology Xla Wt + shh + NF42 (morphology) fig.S3.e^1 Anatomical Phenotype abnormally wholly ventralized eye Larger Image Printer Friendly View

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