Xenbase Image ID: 153360

Fig. S4. Perturbation of S/Hh signaling only affects the floor plate of the brain. Lateral views of tailbud stage control (A, D), cylopamine-treated (B, E), and Shh mRNA injected (C, F) embryos, processed by ISH for F-spondin (A–C) and FoxA2 (D–F) and stained for notochord (Tor70, brown, A–C). Loss of Hh signaling results in reduced (E) or broken (B) staining of floor plate markers in the brain in 48% of cyclopamine-treated embryos (N = 149). Shh mRNA does not cause dorsal expansion of floor plate except in the brain (arrows, C and F). Pink in C, F is staining for co-injected lineage tracer. Image published in: Peyrot SM et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue spon1.S F-spondin X. laevis Throughout NF stage 22 to NF stage 44 brain floor plate eye foxa2.L HNF-3B, hnf3-beta, hnf3b, hnf3beta, LOC108717763 X. laevis Throughout NF stage 22 to NF stage 44 brain floor plate head pharyngeal region Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + Cyclopamine + tailbud stage (in situ hybridization) fig.S4.b Expression Phenotype decreased amount spon1.S expression in eye Xla Wt + Cyclopamine + tailbud stage (in situ hybridization) fig.S4.e Expression Phenotype decreased amount foxa2.L expression in pharyngeal region Larger Image Printer Friendly View

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