Xenbase Image ID: 153361

Fig. S5. Notch and Shh signaling operate in parallel to specify floor plate, but Shh signaling is not required for Notch repression of notochord. Quantification of results shown in Fig. 6. Embryos were injected and/or treated as indicated. At stage 20–22, embryos were fixed, bisected, stained for lacZ lineage tracer, and assayed for FP by Netrin ISH (A, C) and for notochord by Tor70 staining (B). We scored FP development by the intensity of Netrin staining and notochord development by the size of the Tor70+ structure. Total percentages of embryos of each phenotype (see Fig. 6) are represented by stacked column graphs. N is the number of bisected embryos scored in at least 2 independent experiments. For determination of statistical significance, χ2 tests were performed pairwise between 1) controls and single reagents and 2) double treatments and each single component. * significant difference from control, p < 0.05. # significant difference from NICD/SDBM, p < 0.05. + significant difference from other reagent, p < 0.05. Image published in: Peyrot SM et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page