XB-IMG-153472
Xenbase Image ID: 153472
|
|
FIG. 2. Dissection of embryos. The dashed lines indicate the incisions made with glass needles. At stage 12 to 121/2, the neural plate was
dissected without underlying mesoderm (meso); ventral ectoderm (ecto) and endoderm together with mesoderm (labeled endo) were also
collected and cultured for 5 hr at 240C in modified Barth's solution (88 mM NaCI/1 mM KCI/0.8 mM MgCl2/0.8 mM CaCl2/2.4 mM NaHCO3/7.5
mM Tris, pH 7.6) before RNA extraction. At stage 17-19, the closing neural tube was dissected together with the underlying chordamesoderm
(neur). Ectoderm (ecto) was peeled off; the lateral plate mesoderm was collected together with the endoderm (labeled endo). Image published in: Richter K et al. (1988) Copyright © 1988. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |