XB-IMG-153475
Xenbase Image ID: 153475
|
Figure 7. iRECs integrate into reaggregated renal organoids and repopulate decellularized kidneys. (a) A schematic illustration of the reaggregation assay. GFP+ iRECs and E13 TOM+ kidneys were trypsin-digested, mixed in a 1:10 ratio and grown on an air/liquid interface culture. (b) Confocal live-imaging of renal organoids reaggregated with either Ksp-Cre GFP+ iRECs or GFP- transduced control MEFs. The white boxed area is shown enlarged in the right panel. (c) Immunostaining of kidney organoids reaggregated with MEFs or iRECs for the indicated proteins. (d) Tile-scanned confocal images of entire reaggregates after staining for laminin, and enlargement of the white boxed area. (e) Quantification of GFP+ iRECs and MEFs detected inside or outside the laminin-stained basement membrane of reaggregated tubules. Error bars, s.e.m.; student’s unpaired t -test, n = 9 z -stacks, ∗∗∗ P < 0.001. (f) Schematic illustration of the repopulation assay. Wild-type (WT) kidneys were decellularized and injected with iRECs. Confocal images and 3D reconstructions of decellularized kidneys repopulated with Ksp-Cre GFP+ iRECs and immunostained for E-cadherin. Nuclei were stained with Hoechst; ECM, extracellular matrix; MIP, maximum intensity projection of confocal z -stacks. Scale bars, 50 μm (b,c,f), 200 μm (d). Image published in: Kaminski MM et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Macmillan Publishers Ltd. Larger Image Printer Friendly View |