XB-IMG-153476
Xenbase Image ID: 153476
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Figure 8 Reprogramming of human fibroblasts. (a) Phase-contrast image and immunostaining for the indicated proteins in untreated and 4TF- treated human fibroblasts. (b) Relative mRNA expression level of the indicated genes as determined by qPCR. Error bars, s.e.m. Significant differences were assessed by Student’s unpaired t -test, n = 3 biologically independent samples, ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05. (c) Untreated human fibroblasts and human induced renal epithelial cells (h-iRECs) cultured in 3D Matrigel. Shown are maximum intensity projections of confocal z-stacks. (d) Matrigel-grown h-iREC spheres stained for actin (phalloidin) and β-catenin. (e) Percentage of PROM1+ /EPCAM+ double-positive cells (top panel) and percentage of CDH16-GFP+ cells (bottom panel) as determined by flow cytometry. 4TF human fibroblasts were treated with SV40. (f) Heatmap of RNA-seq expression analysis in human fibroblasts (0TF), CDH16-GFP+ sorted h-iRECs (4TF) and human kidney. Differentially regulated genes (0TF versus 4TFs, one-way ANOVA, P < 0.0001, n = 3 independent biological samples) were hierarchically clustered. The number of genes in each cluster is shown on the right (Pearson’s correlation coefficient 0.7). Scale bars, 50μm (a), 100 μm (c), 20 μm (d). Image published in: Kaminski MM et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Macmillan Publishers Ltd. Larger Image Printer Friendly View |