Xenbase Image ID: 153502

Figure 1. Bidirectional Endoderm-Mesoderm Signaling Is Required for Respiratory Specification in the Xenopus Foregut. (A) Model of mesoderm-endoderm paracrine signaling during foregut organogenesis. (B) Schematic of the Xenopus organ induction assay. The foregut endoderm (yellow) and mesoderm (orange) were isolated at NF20 and cultured together or separately until NF36–38. (C) In situ hybridization using the indicated probes reveals that induction of nkx2-1/sftpc+ respiratory progenitors requires the mesoderm, whereas wnt2, wnt2b, and gli1 expression in the mesoderm requires the endoderm (outlines in yellow dashed line in embryo sections). The number of explants expressing the marker is indicated in the lower right of each image. See also Figure S1. Image published in: Rankin SA et al. (2016) Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue wnt2b.L wnt-2b, wnt2b-a, wnt2b-b, Xwnt-2b, xwnt2b X. laevis Throughout NF stage 35 and 36 splanchnic layer of lateral plate mesoderm midbrain-hindbrain boundary lens wnt2.L int1l1, irp, LOC108713107, wnt-2, Xwnt-2, Xwnt2 X. laevis Throughout NF stage 35 and 36 head region splanchnic layer of lateral plate mesoderm midbrain-hindbrain boundary bmp4.L bmp-4, bmp2b, bmp2b1, bmp4-a, bmp4-b, DVR-4, ofc11, XBMP-4, xbmp4, zyme X. laevis Throughout NF stage 35 and 36 head region splanchnic layer of lateral plate mesoderm pharyngeal region gli1.L Gli-1, X-gli1 X. laevis Throughout NF stage 35 and 36 head region splanchnic layer of lateral plate mesoderm pharyngeal region neural tube gli2.L gli4 X. laevis Throughout NF stage 35 and 36 head region splanchnic layer of lateral plate mesoderm pharyngeal region pronephric kidney neural tube gli3.L XGli-3, Xgli3 X. laevis Throughout NF stage 35 and 36 head region splanchnic layer of lateral plate mesoderm neural tube pharyngeal region pronephric kidney shh.L hedgehog, vhh-1, X-SHH, Xhh, xshh X. laevis Throughout NF stage 35 and 36 head region endoderm foregut neural tube pharyngeal region nkx2-1.L nkx2.1, titf1, ttf1, Xnkx-2.1, xnkx2-1 X. laevis Throughout NF stage 35 and 36 forebrain endoderm foregut thyroid primordium lung primordium sftpc.L SP-C, SPC, surfc, xSP-C X. laevis Throughout NF stage 35 and 36 endoderm foregut lung primordium Image source: Published Larger Image Printer Friendly View

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