Xenbase Image ID: 153507

Figure S2 B. relevant to Figure 2. Additional analyses of the Hh/Gli pathway function in Xenopus. (B) In-situ hybridization using the indicated probes on NF34 embryos that were either injected at the 4/8- cell stage into the presumptive foregut mesendoderm with control-MO (20 ng), Gli2+Gli3-MOs (10 ng each), or treated with vehicle or Cyclopamine from NF20-34. Red arrows indicate regions of reduced or absent gene expression in the foregut domain. Loss / severe reduction of ptch1 and gli1 confirms the effective reduction in Hh signaling activity, and co-injection of an RNA encoding an hGli2A (125 pg) rescued lung development confirming the specificity of the Gli2/3-MO depletion. Sox2 immunofluorescence (green) shows abnormal expression of Sox2 in the ventral foregut (boxed area) of cyclopamine-treated and Gli2/3-MO-injected embryos. Abbreviations: lpm; lateral plate mesoderm. The number of embryos exhibiting the phenotype is indicated in the lower right of each panel. Image published in: Rankin SA et al. (2016) Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Experiment + Assay Source Phenotypes and Disease Xla Wt + Cyclopamine + NF33/34 (immunohistochemistry) Fig S2 B Expression Phenotype decreased amount gli1 expression in lateral plate mesoderm decreased amount wnt2b expression in lateral plate mesoderm Xla Wt + gli2 MO + gli3 MO + NF33/34 (in situ hybridization) Fig S2 B Expression Phenotype decreased amount nkx2-1 expression in forebrain decreased amount nkx2-1 expression in foregut endoderm decreased amount nkx2-1 expression in lung primordium decreased amount nkx2-1 expression in thyroid primordium decreased amount wnt2 expression in lateral plate mesoderm Larger Image Printer Friendly View

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