Xenbase Image ID: 153508

Figure S2 C relevant to Figure 2. Additional analyses of the Hh/Gli pathway function in Xenopus. (C) Pharmacological disruption of the Hh and RA pathways results in a loss of Wnt/bcatenin reporter activity in the ventral foregut endoderm. Transgenic X. tropicalis Tg(7xtcf:eGFP) embryos were treated with cyclopamine from NF20-34 or with BMS493 (pan-RAR antagonist) or DEAB (aldehyde dehydrogenase inhibitor) from NF15 to 25. Wnt-reporter activity was assayed at NF34 by GFP immunofluorescence (green). Sections were co-stained with Raldh2 (blue) and Nkx2-1 (red) antibodies, indicating the lpm and respiratory epithelium respectively. White boxed area indicates the ventral foregut. Image published in: Rankin SA et al. (2016) Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page