XB-IMG-153509
Xenbase Image ID: 153509
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Figure S2 D, E. relevant to Figure 2. Additional analyses of the Hh/Gli pathway function in Xenopus. (D) Quantification of mitotic cell number by phospho-Histone H3 (pHH3) staining in control and HH pathway-disrupted embryos at NF35. The average number of pHH3+ cells observed in either the endoderm (yellow bars), or mesoderm (pink bars) were quantified from 30 μM confocal z-stacks of the foregut (n=5 embryos per condition). Error bars +/- standard deviation; * p<0.05 in student t-test relative to controls. Abbreviations: cyclo, cyclopamine.
(E) Quantification of apoptosis by activated caspase-3 (casp-3) immunofluorescence in control and HH pathway-disrupted embryos at NF41. The average number of casp-3+ cells in either the endoderm (yellow bars) or mesoderm (pink bars) were quantified from 30 μM confocal z-stacks of the foregut (n=5 embryos per condition). Error bars +/- standard deviation; * p<0.05 in student t-test relative to controls. Abbreviations: cyclo, cyclopamine. Image published in: Rankin SA et al. (2016) Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |