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Figure S4A relevant to Figure 4. Additional analyses of RA loss-of-function in Xenopus.
(A) In situ hybridization of RA pathway components at stages NF15, NF20, and NF34. For stages NF15 and 20 sagittal and transverse views of bisected embryos are shown. Dashed white line on the sagittal view shows plane of section shown in the transverse view. For NF34, white dashed line indicates plane of section shown; yellow dashed line outlines the endoderm. Expression of raldh2 is restricted to the mesoderm at all stages examined, whereas rar alpha is detected in both mesoderm and endoderm at all stages examined. Rar gamma is expressed in the anterior-most endoderm and mesoderm at NF15 and NF20, however is down-regulated in the foregut by NF34. Abbreviations: lpm, lateral plate mesoderm; end, endoderm; ae, anterior endoderm; fge, foregut endoderm.
