Xenbase Image ID: 153518

Figure S4E relevant to Figure 4. RA has temporally distinct functions in Xenopus lung development. (a,b) A schematic of the experimental design. Intact foregut explants (endoderm + mesoderm) were dissected at NF14 and cultured as describe in (b) until NF38. In-situ hybridization of foregut explants at NF38 shows that BMS493 treatment from NF15-25inhibits expression of nkx2-1 and wnt2b; expression of these genes cannot be rescued by culture with exogenous FGF10. In contrast BMS493 treatment from NF25-35 reduces expression of nkx2-1, wnt2b and inhibits expression of sftpc; however exogenous FGF10 is able to rescue expression of these genes when added during the NF25-38 time period. The two temporal roles for RA signaling are diagrammed in (c). Image published in: Rankin SA et al. (2016) Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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