Xenbase Image ID: 153523

Figure 7. RA Is Required for the Respiratory Competence of Human Foregut Endoderm Cultures (A) Schematic depicting the differentiation protocol of H1 hESCs to test the role of RA signaling in respiratory competence of human foregut endoderm culture. (B) RT-qPCR analysis shows that NKX2-1 is robustly induced by CHIR + BMP4 (d6–9) when cultures were treated with RA on d5–6. SOX2 and FOXA1 confirm foregut identity (∗p < 0.05 in Student’s t test of control versus RA treated, n = 4). (C) Immunofluorescence of d9 cultures reveals widespread expression of NKX2-1 in response to the RA/CHIR/Bmp4 treatment. The boxed insets show a 40× magnification. Image published in: Rankin SA et al. (2016) Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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