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Fig. 1. Transcriptome analysis of animal explants induced by co-injection of wnt3a and chd mRNA. (A) Schematic diagram of experimental procedures. (B) Kctd15 suppressed the expression of NC marker genes in animal cap assays. Both blastomeres of two-cell stage embryos were injected with the indicated mRNAs. RT-PCR was performed to examine expression of snai2, sox9, and foxd3. Co-overexpression of wnt3a and chd strongly induced the expression of these genes, and the induction was suppressed by addition of kctd15. Odc was the loading control. WE, whole embryos. (C,D) The top ten enriched GO terms in differentially expressed genes in the comparison of (B) induced to control (WC/L), and (C) induced/inhibited to induced (WCK/WC) animal caps. Image published in: Wong TC et al. (2016) Copyright © 2016. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

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