XB-IMG-153814
Xenbase Image ID: 153814
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Fig. 4. XSu(H)2 directly bound to the upstream region of Xoct25 regulated its transcription. (A) Comparison of upstream sequences of Xoct25 between
Xenopus laevis and tropicalis. Gray boxes indicate the high homology of S1 and S2 in the SPS motif. (B) Schematic drawings of several deletion forms in
Xoct25 reporter construct. The upstream region of Xoct25 ( 1042) truncated with S1 (DS1), S2 (DS2) or both (DS1/DS2) was fused to the luciferase
reporter gene. Adenine at the first methionine is defined as +1. (C) Effect of SPS motif mutation on reporter activity. All values were standardized by
calculating luciferase activity in the upstream of Xoct25 ( 1042) as 1. Experiments were carried out in triplicate. (D) Two nanograms MT-XSu(H)2 into
both blastomeres of two-cell stage embryos, the injected embryos were cultured until stage 10.25 for ChIP assay. A sample prepared from embryos without
injection was used as ChIP control. Anti-myc, precipitation using anti-myc antibody; no Ab, precipitation without antibody; input, cross-linked chromatin
supernatant before immunoprecipitation. Image published in: Ito M et al. (2007) Copyright © 2007. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |