XB-IMG-153857
Xenbase Image ID: 153857
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Fig. 2. ASPN induces eye-like structures.
(A,B) Arrows indicate ectopic eye-like structures that
appeared following overexpression of ASPN
mRNA (B). (C-H) Injection of ASPN mRNA induces
an eye-like structure. In contrast to the embryos
injected with 3 ng of control (β-Galactosidase)
mRNA (C-C′,F), either 1 ng (D-D′,G) or 3 ng
(E-E′,H) of ASPN mRNA at the 4-cell stage induced
ectopic pigmented structures (arrows) at stage 42.
The whole structure was imaged (C-E′), or
Haematoxylin and Eosin staining was performed
with sectioned samples (F-H). (I) Quantification of
the phenotypes induced by injection of ASPN mRNA
at various concentrations. The phenotypes were
divided into four categories: embryos with normal
eyes, with enlarged eyes (as in D-D′), with
ectopic eyes (as in E-E′) and with a short axis.
(J-O) Immunohistochemistry performed on sections
of the pigmented eye-like structure induced by
ASPN show that it contains eye-specific
components. Embryonic eyes (J,L,N) and the
pigmented structure induced by injection of ASPN
(K,M,O) were analysed at stage 42 with β-Crystallin
(J,K), Glutamine Synthetase (L,M) and Hu-C/Hu-D
(N,O) antibodies. Green, immunohistochemical
signal; blue, DAPI. (P) The phenotypes found
following injection of SLRP family members. SLRP
family members were injected at 3 ng into a dorsal
animal blastomere at the 4-cell stage and the
phenotypes were categorised at stage 42. Key is
shown in I. Image published in: Luehders K et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |