Xenbase Image ID: 153858

Fig. 3. ASPN induces forebrain marker genes both in vivo and in vitro. (A-L) Forebrain marker genes were increased at the expense of posterior markers in vivo. The tracer β-Galactosidase (light blue product) was injected without (A,C,E,G,I,K) or with (B,D,F,H,J,L) ASPN mRNA and embryos were analysed by in situ hybridisation with Rx (A,B), Pax6 (C,D), Otx2 (E,F), FoxG1 (G,H), En2 (I,J) or Krox20 (K,L) probes at stage 18. Affected areas are indicated by arrowheads. (M) Control (lane 2) or ASPN-injected (lane 3) animal cap explants were analysed by semi-quantitative RT-PCR. Whole embryos (lane 1) were used as a positive control for the PCR. Image published in: Luehders K et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue rax.L rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 18 optic vesicle eye primordium pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 18 neural plate pre-chordal neural plate chordal neural plate eye primordium optic vesicle otx2.L otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 18 neural plate pre-chordal neural plate anterior placodal area optic vesicle eye primordium foxg1.L bf1, foxg1b, LOC108700624, XBF-1, XBf1 X. laevis Throughout NF stage 18 neural plate pre-chordal neural plate cranial placode neurogenic placode en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 18 presumptive midbrain-hindbrain boundary egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 18 presumptive rhombomere Image source: Published Larger Image Printer Friendly View

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