Xenbase Image ID: 153864

Figure S3. Designation of morpholino oligonucleotides against ASPN and the phenotypes caused by the ASPN-MO2. (A) In addition to ASPNa, which this study is based on, we found another genome sequence probably due to the pseudotetraploidity, and termed it ASPNb. The nucleotide sequences (black characters) around the start codon (circled) of Xenopus ASPN and the sequences of ASPN-MO1 (red) ASPNa-MO2 (blue) and ASPNb-MO2 (purple) are shown. (B-D) Representative images from the injection of 20 ng control-MO (B), 20 ng ASPN-MO2 (C) and 20ng ASPN-MO2 together with 1 ng of the coding region of ASPN (ASPNCDR) mRNA (D). (E) Quantification of the phenotypes. For the rescue experiment embryos were injected with either 20 ng ASPN-MO2 and 1 ng ASPNCDR, or 20 ng ASPN-MO2 and 3 ng ASPNCDR and the phenotypes analysed at stage 41. Image published in: Luehders K et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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