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Figure S4. Both IGF and ASPN are required for the full activation of ERK. Animal cap explants were prepared from 3 ng control -Galactosidase (i,ii,iv), 3ng dnIGFR mRNA (iii), 20 ng control-MO (v) or 20 ng ASPN-MO (vi) injected embryos and were incubated with the conditioned media expressing control (i,iv), ASPN (ii,iii) or IGF2 (v,vi) for 20 minutes. The explants were analysed by western blotting using phosphor-ERK or ERK antibodies. Image published in: Luehders K et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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