XB-IMG-153883
Xenbase Image ID: 153883
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Figure S7. Validation of Lhx9 depletion assays.
(A) Validation of MO-specific inhibition of Lhx9 translation by GFP western blot on stage 11 embryos, injected with 1ng Lhx9-5’UTR-GFP RNA and MO at various concentrations (20-40 ng). Shp2 is used as a protein loading control. MOT targets the translational start site from both the short and long genomic versions of X. laevis Lhx9. (B) RT-PCR analysis of cardiac cDNA from stage 42 embryos injected with both MO1 (30ng each) targeting the short and long genomic versions of X. laevis Lhx9. Negative control lanes (-) are without superscript II enzyme, GAPDH PCR as loading control. Image published in: Tandon P et al. (2016) Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |