Xenbase Image ID: 153900

Fig. 1. Wdpcp controls plasma membrane stability in epithelial cells. (A) Control tissue expressing membrane-RFP, cell–cell contact indicated by the blue box is shown in (a0 ). (B) Morphant tissue showing severe membrane blebbing. The boxed region is shown in (b0) with higher magnification. Asterisks indicate blebs. (C and c0) Co-injection of Wdpcp-MO and Wdpcp mRNA (300 pg) rescued the membrane blebbing. (D) The quantification of membrane blebbing. Blebbing index was determined as the ratio of total blebbing area within a single cell-contact compared to the length of that cell–cell contact. Control n = 125; Wdpcp-MO n = 196; Rescue n = 131. The scale bars are 10 lm. (E) Still frames from a time-laps movie of control epithelium (Supplemental time laps Movie 1). (F) Still frames from a time-laps movie of Wdpcp morphant epithelium (Supplemental time laps Movie 2). Image published in: Park TJ et al. (2015) Copyright © 2015. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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