Xenbase Image ID: 154018

Figure 2. nup188 and nup93 Depletion Decreases Cilia in the LRO (A) LROs from embryos treated with the indicated MOs (or UC) stained with anti-acetylated α-tubulin (α-AcTub, green) to detect primary cilia, Phalloidin (red) marks the cell borders. p, a, l, and r denote posterior, anterior, left, and right, respectively. Right panels show a magnification of a representative region of the LRO. (B) Box plots of cilia number per LRO area (μm2) of embryos derived from injections at the 1- or 2-cell stage. Box depicts 25th to 75th percentiles with median marked by a horizontal line in the box. Whiskers indicate data range from smallest to largest values. n represents total number of embryos from three independent experiments. ∗∗∗p < 0.0005, ∗∗p < 0.005, Student’s t test. Image published in: Del Viso F et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue actb beta actin, beta-actin, g-actin X. tropicalis Sometime during NF stage 28 to NF stage 31 gastrocoel roof plate ciliated cell tuba4b alpha tubulin, alpha-tubulin, tuba4, tuba4a X. tropicalis Sometime during NF stage 28 to NF stage 31 gastrocoel roof plate monociliated cell cilium ciliary axoneme Image source: Published Experiment + Assay Source Phenotypes and Disease Xtr Wt + nup188 MO + NF16 (immunohistochemistry) Fig. 2 A B Anatomical Phenotype abnormally decreased number of cilium in the left-right organizer Disease congenital heart disease visceral heterotaxy Xtr Wt + nup188 MO + NF16 (immunohistochemistry) Fig. 2 A B Anatomical Phenotype abnormally decreased number of cilium in the left-right organizer Disease congenital heart disease visceral heterotaxy Xtr Wt + nup188 MO + NF16 (immunohistochemistry) Fig. 2 A B Anatomical Phenotype abnormally decreased number of cilium in the left-right organizer Disease congenital heart disease visceral heterotaxy Xtr Wt + nup93 MO + NF16 (immunohistochemistry) Fig. 2 A B Anatomical Phenotype abnormally decreased number of cilium in the left-right organizer Disease congenital heart disease visceral heterotaxy Xtr Wt + nup93 MO + NF16 (immunohistochemistry) Fig. 2 A B Anatomical Phenotype abnormally decreased number of cilium in the left-right organizer Disease congenital heart disease visceral heterotaxy Xtr Wt + nup93 MO + NF16 (immunohistochemistry) Fig. 2 A B Anatomical Phenotype abnormally decreased number of cilium in the left-right organizer Disease congenital heart disease visceral heterotaxy Larger Image Printer Friendly View

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