Xenbase Image ID: 154021

Figure 5. Nups Do Not Form NPC-like Rings at Cilia Bases (A) 2D FPALM super-resolution image of a cell co-stained with anti-Nup93 (red; AF647) and anti-AcTub antibodies (green; Cy3B). (B) Higher-magnification views of the nuclear surface (left panels) and the cilium base (right panels) of two different cells stained with anti-Nup93 (AF647). (C) Box plot where the density of localization clusters at the nuclear envelope and cilia bases are directly compared. The box depicts the 25th to 75th percentiles with the median marked as a horizontal line in the box. The whiskers mark the data range from the smallest to the largest value. “+” indicates the mean of the distribution (n represents number of cells). ∗∗p < 0.005, Student’s t test. (D) Plot of the diameter (mean ± SD) of localization clusters at the nuclear envelope and cilia bases. n represents number of localization clusters from three independent experiments. ∗∗∗p < 0.0005, Student’s t test. (E) 2D FPALM images of anti-Nup188 (red; AF647) and anti-γTub antibodies (green; Cy3B) with merge on right. (F) As in (E) except with anti-AcTub co-stain. (G) STED images of a cell co-stained with anti-Nup188 (red; ATTO647N) and anti-γTub antibodies (green; ATTO594) with merge on right. (H) As in (G) except with anti-AcTub co-stain. See also Figure S4. Image published in: Del Viso F et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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