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Fig. 10. Further characterization of a later generation of nop. (A, B) A different pigmentation mutation additively affects the nop pigmentation phenotype. A double mutant for nop and an axanthic gray color mutant line ICB (A) and a nop single mutant (B) (which could be heterozygous or wild-type at the ICB locus) in stock 614. We obtained offspring of this population and compared eye size with wildtype. (C) Illustration of the axis and boundary used for eye diameter measurement. (D) Box-and-whisker plot representing the distribution of eye size in wildtype (+/+) and nop (/) tadpoles. The middle line shows the median value, with the box representing the 25th and 75th percentiles and the whiskers extending to the minimum and maximum observed values. The asterisk (*) indicates a statistically significant difference between two populations. Statistical comparison of wildtype (n=10, mean=338 m, SD=18 m) and nop (n=10, mean=322 m, SD=11 m) eye diameters yields a p-value of 0.03 (two-tailed t-test). Image published in: Nakayama T et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V.
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