Xenbase Image ID: 154066

Fig. 2. Tbx2 acts as a target gene of BMP pathway to inhibit FGF signaling. (A) Phenotypes of embryos injected animally with Tbx2 (200 pg), Tbx2MO (10 ng) or FGF8 (200 pg) or treated with SU5402 (20 μM) from the four-cell stage to tadpole stage. Control, an uninjected control embryo. (B-G) Animal caps from the embryos injected animally with the indicated combination of FGF8 (1 ng), v-Ras (50 pg), Tbx2 (B, 200 pg; D, 100–200 pg), chrodin (200 pg), noggin (10 pg), BMP4 (200 pg), Tbx2 MO (10 ng) and CO MO (10 ng) were cultured to stage 12 for western blotting (B, C, F, G) and to stage 15 for RT-PCR analysis (D, E). (C) and (G) show quantification of the levels of p-ERK (normalized to ERK) for (B) and (F), respectively. Error bars indicate the standard error (SE) from three independent experiments. Asterisks above the bars denote *p-value <0.05 and **p-value <0.01. ODC serves as a loading control. WE, whole embryo. Con. AC, uninjected control animal caps. –RT, a control in the absence of reverse transcriptase. Image published in: Cho GS et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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