Xenbase Image ID: 154073

Fig. S2. Expression of PSC genes increases in embryos with disabled Notch signaling. (A-F) Whole-mount in situ hybridization of stage 26 tadpoles that were injected on one side at the 2-cell stage with RNA encoding HMMmut to block Notch signaling in the developing skin, along with RNA encoding β-galactosidase as a tracer. The injected side was identified by X-gal staining (light blue) and RNA expression was revealed by NBT/BCIP staining (light purple). Shown is the uninjected (left panel) and injected side (right panel) for transcripts encoding foxi1 (A), two subunits of the H+v-ATPase involved in proton transport (B,D), an isoform of carbonic anhydrase (C), pendrin-like (E) and the ciliated marker α-tubulin as a control (F). Image published in: Quigley IK et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page