Xenbase Image ID: 154080

Fig. 9. foxi1-HGR induces ae1 and alpha-INCs. (A-D) Embryos were injected at the 2-cell stage with foxi1-HGR, treated with DEX at stage 12 and then processed for ae1 and pendrin expression by whole-mount in situ hybridization. Shown are representative examples of uninjected and injected regions. Note that foxi1-HGR induces a massive increase in ae1 expression, but only a small increase in the number of cells expressing pendrin. (E,F) Example of a control transplant (E) stained with antibody to ae1 (F). (H,I) Example of a transplant onto a host injected with foxi1-HGR RNA (H) and stained with ae1 antibodies. The arrowhead marks ae1-negative INC (I). (G) Transplant assay where the outer layer is removed from ectoderm at stage 10 from a mGFP-injected host and placed on the inner layer of a host injected with mRFP or with both foxi1-HGR and mRFP RNA. INCP, intercalating non-ciliated cell precursor; CCP, ciliated cell precursor. (J) Quantification of ae1-positive and -negative INCs in control or foxi1-HGR injected hosts (three random fields from at least eight embryos per condition). Error bars indicate ± s.d. All values between a given INC subtype under the various conditions are significantly different relative to the control, based on a two-tailed t-test (P≤0.005). Scale bar: 20 µm. Image published in: Quigley IK et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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