Xenbase Image ID: 154081

Fig. 1. MD3 structure and expression in Xenopus embryo. (A) Xenopus laevis MD3 (MD3; Genbank: BC 068841) protein includes three intracellular (IC, red), four transmembrane (TM, green) and two extracellular (EC, blue) domains. Numbers correspond to the amino acids. Spatial analysis of expression of MD3 transcript (B,D,D′) and an eye marker Rx1 (C,E) was performed by whole-mount in situ hybridization (WISH) in neurulas (stage 15, B) and tadpoles (stage 38, D,D′). Scale bar: 500 μm; black arrowhead, eye; white arrowhead, lens. (F) MD3 expression analysis by semi-quantitative RT-PCR using RNA isolated from dissected optic vesicles (stage 25) and eyes (stage 42). GAPDH was amplified as a control. bp, base pairs. (G) MD3 signal was detected by WISH in the inner and outer plexiform layers (red arrowheads) and in the lens (white arrowhead) on eye sections (stage 42). Image published in: Vacca B et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue marveld3.L md3 X. laevis Throughout NF stage 15 eye primordium anterior placodal area marveld3.L md3 X. laevis Throughout NF stage 37 and 38 lens eye retina head mesenchyme intersomitic region rax.L rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 15 eye primordium anterior placodal area rax.L rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 37 and 38 eye retina intersomitic region Image source: Published Larger Image Printer Friendly View

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