Xenbase Image ID: 154083

Fig. 3. MD3 overexpression changes eye lamination. Eye morphology was analysed (A-B′) and quantified (C) in non-injected (NI), GFP or FL MD3 mRNA-injected embryos (P values in C, GFP RNA=0.89; FL MD3 RNA=0.26; NS: non significant). Lateral (A,B) and dorsal view (A′,B′) of the embryo; GFP mRNA was used as a control. Red asterisk, injected side of the embryo. (D,E), The eye size was quantified for GFP or FL MD3 mRNA-injected embryos, as described in the schemes and in the Materials and methods. Numbers indicate the embryos analysed; NS, non significant. P values in D, GFP RNA=0.66; FL MD3 RNA=0.91; P values in E, GFP RNA=0.46; FL MD3 RNA=0.52; NS, non significant. (F,G) Histological analysis by H&E staining was performed on eye sections from GFP and FL MD3 mRNA-injected embryos and the number of cells was quantified as described in the Materials and methods. (H) Number of cell were quantified per eye section stained by H&E derived from GFP or FL MD3 RNA-injected embryos. Numbers indicate the eye sections quantified; P value=1.26*10−6, ***P<0.001. Immunohistochemistry on eye sections from GFP and FL MD3 mRNA-injected embryos was perfomed for β-crystallin (I-I′), rhodopsin (J,J′), Hu-C/Hu-D (K-K′). (L) Number of positive cells for each eye marker was quantified as described in the Materials and methods. Numbers indicate the eye sections quantified; P values, β-crystallin=0.19; Rhodopsin=0.87; Hu-C/Hu-D=0.74; NS, non significant. Statistical significance was assessed by Mann–Whitney (C,L) and Student's t-test (D,E,H). Scale bars: 500 µm (A,B) and 100 μm (I-K). Embryos were analysed at stage 42. Image published in: Vacca B et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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