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Fig. 6. MD3 KD stimulates cell proliferation and apoptosis in the eye field. Eye-field proliferation was analysed by WISH against cyclinD1 in neurula (stage 17; anterior view; A-A′) and tailbud (stage 30; B-B′) injected with CT or MD3 MOs. Black dotted line, midline of embryo; arrowhead, enlarged (A′) and reduced (B,B′) expression domain of cyclinD1; red asterisks, injected side of the embryo; numbers indicate the embryos analysed. Scale bar: 500 µm. Cell proliferation in the anterior region of the neurula was quantified with a BrdU staining (C) and cell death with a TUNEL assay in tailbud (D) as described in the Materials and methods; Student's t-test (P values, BrdU in C, CT MO=0.16, MD3AB MO=9.4*10−6; TUNEL in D, CT MO=7*10−2, MD3AB MO=5.66*10−9). The numbers indicate the embryos analysed (A-B′,D) or the sections counted (C). NS, non significant; ***P<0.001. Image published in: Vacca B et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue ccnd1.L ccnd1-a, ccnd1-b, cyclind1, parathyroid adenomatosis 1, prad1 X. laevis Throughout NF stage 17 eye primordium anterior placodal area anterior neural ridge pre-chordal neural plate anterior neural fold pre-chordal neural plate border ccnd1.L ccnd1-a, ccnd1-b, cyclind1, parathyroid adenomatosis 1, prad1 X. laevis Throughout NF stage 29 and 30 eye retina optic vesicle retinal layer midbrain-hindbrain boundary branchial arch Image source: Published Larger Image Printer Friendly View

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