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Fig. 9. MD3-induced JNK activation is required for eye morphogenesis. (A) Activity of the reporter plasmid AP1-Luc (JNK pathway) was determined by Luciferase assay from whole-embryos (stage 12) co-injected with Renilla (as a control), c-Jun (as positive control) or FL MD3 mRNA. The morphology of the eye was analysed at stage 42 (B-C′), quantified (D) and the eye size determined (E) in embryos injected with MD3 MOs, co-injected with wt JNK or CA-JNK mRNA. Scale bars: 500 µm. The numbers indicate the embryos analysed. Statistical significance was assessed by Student's t-test (P values in A, positive CT=2.6*10−3; MD3 RNA=3.5*10−3) and Mann–Whitney test (P values in D, MD3AB MO=0.02; MD3AB MO+wt JNK RNA=0.06; MD3AB MO+CA-JNK RNA=0.19; P values in E, MD3AB MO=1.65*10−9; MD3AB MO+wt JNK=1.4*10−3; MD3AB MO+CA-JNK=4.37*10−6). *P<0.05; **P<0.01; ***P<0.001; NS, non significant. Image published in: Vacca B et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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