Xenbase Image ID: 154093

Fig. 4. CRMM disruption enhances the Wnt/β-catenin signaling pathway activation. (A) RT-PCR showing expression of wnt3a and target genes myf5 and myoD increased in whole embryos and dissected head anlage. (B) TOP-FLASH reporter activation is increased by cholesterol depletion at stage 13 but not at stage 10. (C) RKO-cells showing Wnt signaling activity enhanced by MβCD in synergism with Wnt conditioned medium. (D, E) Western blotting showing higher levels of β-catenin and CyclinD1 after cholesterol depletion. Co (control), M (MβCD), W (Wnt), W + M (Wnt conditioned medium and treated with MβCD). (F) L-cell conditioned medium enhances Wnt activity in RKO-cells after cholesterol depletion. Co (conditioned medium from L-cell non-secreting Wnt), Wnt (conditioned medium from Wnt-secreting L-cell), W + V (conditioned medium from Wnt-secreting L-cell treated with vehicle), W + M (conditioned medium from Wnt-secreting L-cell treated with MβCD). (G) Western blotting showing increase in total Wnt protein from conditioned medium after L-cell producing Wnt MβCD treatment. Coomassie blue stained gel after electrophoresis showing loaded proteins. Statistical significance: **P < 0.01. Image published in: Reis AH et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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