Xenbase Image ID: 154131

Fig. 5. c-Myc can rescue the effect of Cdk and CyclinT1 morpholino knockdown (A) Embryos were co-injected into one cell of a two cell stage embryo with a morpholino and c-Myc RNA. 300 pg of Lac-Z cRNA was co-injected with morpholinos and RNA. β-gal activity was detected using a red gal stain to monitor lineage tracing. Embryos then underwent Sox10 in situ. Injection of 100 ng Cdk9.S morpholino and 60 ng CyclinT1 morpholino results in a loss of Sox10 expression on the injected side. Co-injection of Cdk9.S and CyclinT1 morpholino with 100 pg c-Myc RNA results in a partial rescue of Sox10 expression. Co-injection of Cdk9.S and CyclinT1 morpholino with 500 pg c-Myc RNA results in a greater rescue of Sox10 expression. Scale bar=200 µm. These results are quantified in Fig. S6D. (B) Graph showing the percentage of embryos displaying an expansion, wild type, partial loss or loss of Sox10 expression pattern by in situ hybridisation after injection of Cdk9.S morpholino alone (n=51) and co-injected with 100 pg (n=114) and 500 pg of c-Myc RNA (n=61). CyclinT1 morpholino alone (n=46) and co-injected with 100 pg (n=61) and 500 pg of c-Myc RNA (n=52). 500 pg of c-Myc RNA alone (n=34) and 100 ng of a standard control morpholino alone (n=33). Image published in: Hatch VL et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue sox10.L X. laevis Sometime during NF stage 15 to NF stage 16 neural plate border neural crest Image source: Published Larger Image Printer Friendly View

Return to previous page